Extended Data Fig. 8: Inflammatory response in wild type and SQS-KO BMDMs challenged with LPS.
From: Microglia facilitate repair of demyelinated lesions via post-squalene sterol synthesis
a, Mean ROS levels in control (left) and SQS-KO BMDMs (right) stimulated with or without LPS and treated with or without squalene (100 µM) (n = 8 cultures per condition). Data normalized to untreated control (set to 1). Asterisks mark significant changes (two-way ANOVA with Holm-Sidak post test). b-f, Relative expression of cholesterol efflux (b, c) and inflammatory (d-f) genes in control and SQS-KO BMDMs treated with myelin+LPS with or without squalene (as depicted in Fig. 5c), normalized to untreated WT cultures(n = 4 cultures) (two-way ANOVA with Holm-Sidak post test). Asterisks mark significant changes, ***p < 0.001, **p < 0.01, *p < 0.05.
