Extended Data Fig. 1: Gel Autoradiography of I-S1 from RayBiotech and AMSBIO.
From: The S1 protein of SARS-CoV-2 crosses the blood–brain barrier in mice
Cropped images of Fraction 9 of I-S1 from RayBiotech (left panel, 1ul of approximately 300,000 CPM run under reducing and non-reducing conditions, exposed to film for 24 hours), and from AMSBIO (right panel, 1ul of approximately 600,000 CPM run under reducing and non-reducing conditions. In each case, both the reduced and non-reduced gels migrated at the molecular weights predicted by the manufacturers. The autoradiography gels to validate size were run once. The quality of subsequent I-S1 labeling was confirmed by acid precipitation, described in methods.
