Extended Data Fig. 7: Intrainfarct application of AMD3100 induces ectopic positioning of MDM after PT.

Cxcr4^CreER/Wt; R26^CAG-LSL-tdT mice received saline or AMD3100 into the infarct at day 8 after PT via stereotactically-controlled injection. Images were captured 6 h after substance application. a, Counterstained tdT and GFAP. Regions of interest (ROIs) used to quantify the presence of MDM are illustrated (ROI#1: infarct, ROI#2: peri-infarct, ROI#3: intact region). b, Iba1 staining (green) is shown for cells labeled with arrows in a. DAPI appears white. The depicted tdT⁺ cells in the saline-treated animal are Iba1⁻ presumptive neurons. The tdT⁺ Iba1⁺ cell in the AMD3100-treated animal represents an ectopic MDM in ROI#3. a, b, Images are representative for n = 6 each. Scale bars: 200 µm (a), 20 µm (b).