Extended Data Fig. 1: Analysis of Cxcr4-GFP expression. | Nature Neuroscience

Extended Data Fig. 1: Analysis of Cxcr4-GFP expression.

From: Cxcr4 distinguishes HSC-derived monocytes from microglia and reveals monocyte immune responses to experimental stroke

Extended Data Fig. 1

a, Flow cytometry analysis of GFP signal in postnatal ( > P21) Cxcr4-GFP mice (solid line histograms) and wild-type controls (filled histograms). Cells analyzed were blood leukocytes including B-cells (CD19+), T-cells (CD3+), natural killer cells (NKp46+), neutrophils (Ly6G+), Ly6Clow and Ly6Chi monocytes (CD115+ Ly6Clow / Ly6Chi), CD11b+ F4/80high tissue MΦ (gate 1), and CD11bhigh tissue myeloid cells (gates 2 and 3). Data are representative for n = 4 (blood), n = 7 (spleen, kidney, and liver), and n = 6 (epidermis) mice. b, Confocal micrographs of immunofluorescences for GFP (green) and F4/80 (magenta) in the indicated tissues of postnatal Cxcr4-GFP mice ( ≥ P28). Arrowheads point to Cxcr4-GFP+ F4/80− and arrows to Cxcr4-GFP− F4/80+ cells. Representative for n = 3 mice each. Scale bars: 45 µm (b).

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