Extended Data Fig. 5: Histology and 3D rendering of LHb GAD2 neurons and orexin axons.
From: Orexin signaling in GABAergic lateral habenula neurons modulates aggressive behavior in male mice
a, Immunohistochemistry for orexin-A (red), DAPI (blue), and eGFP (green) in a GAD2-Cre mouse injected with AAV-DIO-eGFP, scale bar = 300 μm. Experimental images were taken from 3 biologically independent mice, 3 slices per mouse, with similar results obtained. b, Immunohistochemistry for orexin-A (red), DAPI (blue), and GFP (green) in a GAD2-Cre mouse injected with AAV-DIO-eGFP, scale bar = 10 μm. Experimental images were taken from 3 biologically independent mice, 3 slices per mouse, with similar results obtained. c, 3D rendering of image in b, color of GAD2 neuron coincides with estimated distance from orexin-A axon according to key in lower right corner, scale bar = 5 μm. Experimental images were taken from 3 biologically independent mice, 3 slices per mouse, with similar results obtained.
