Extended Data Fig. 7: Effects of systemic antagonism of OxR2 with EMPA on aggression and LHb activity.
From: Orexin signaling in GABAergic lateral habenula neurons modulates aggressive behavior in male mice
a, Experimental scheme for OxR2 systemic antagonism RI experiment. b, RI test attack latency in animals treated with EMPA and vehicle (two-tailed paired t-test, n = 11 biologically independent mice per group, t(10)=0.3215, p = 0.758). c, RI test attack duration in animals treated with EMPA and vehicle (two-tailed paired t-test, n = 11 biologically independent mice per group, t(10)=2.888, p = 0.016).d, Experimental scheme for OxR2 systemic antagonism aggression CPP and locomotion experiments. e, Aggression CPP for animals treated with EMPA and vehicle (two-tailed student’s t-test, n = 12 biologically independent vehicle mice and n = 11 biologically independent EMPA mice, t(21)=2.885, p = 0.0086). f, Locomotor activity in the open field for animals treated with EMPA and vehicle (two-tailed student’s t-test, n = 12 biologically independent vehicle mice and n = 11 biologically independent EMPA mice, t(21)=0.1301, p = 0.8991). g, Anxiety-related behavior in the open field for animals treated with EMPA or vehicle (two-tailed student’s t-test, n = 11 biologically independent mice per group, t(21)=1.134, p = 0.2695) h, Representative fiber photometry traces in an animal treated with vehicle and EMPA. i, LHb GCaMP peaks during RI during vehicle and EMPA treatment (two-tailed paired t-test, n = 5 biologically independent mice, t(4)=2.946, p = 0.0421). *p < 0.05, **p < 0.01. All data are expressed as mean ± SEM.
