Extended Data Fig. 8: LHb orexin-ChR2 experiments supporting data.
From: Orexin signaling in GABAergic lateral habenula neurons modulates aggressive behavior in male mice
a, >90% of neurons infected with AAV1-DIO-YFP were positive for orexin-A protein as determined by immunohistochemistry, n = 3 biologically independent mice, 3 slices per mouse. b, Surgical manipulations for ChR2-mediated activation of orexin terminals in the LHb with concurrent knockdown of LHb OxR2. c, Optogenetic stimulation of orexin terminals in the LHb reduced attack latency in mice treated with the miR-scrambed virus, but not the miR-OxR2 virus (two-tailed paired t-test, miR-scrambled: n = 11 biologially independent mice, t(10)=2.424, p = 0.0358; miR-OxR2: n = 9 biologically independent mice, t(8)=0.5281, p = 0.6117). d, Optogenetic stimulation of orexin terminals in the LHb increased attack duration in mice treated with the miR-scrambled virus, but not the miR-OxR2 virus (two-tailed paired t-test, miR-scrambled: n = 11 biologically independent mice, t(10)=2.260, p = 0.0474; miR-OxR2: n = 9 biologically independent mice, t(8)=0.8493, p = 0.4204). *p < 0.05. All data are expressed as mean ± SEM.
