Extended Data Fig. 10: In-vitro and in-vivo validation of AAV-DIO-OxR2 over-expression virus.
From: Orexin signaling in GABAergic lateral habenula neurons modulates aggressive behavior in male mice
a, N2A cells treated with OxR2 over-expression construct selectively increased OxR2 expression compared to controls (two-tailed student’s t-test, n = 3 biologically independent plates per group, 3 replicates per plate, OxR2: t(4)=3.939, p = 0.0171; OxR1: t(4)=0.1238, p = 0.9075). b, GAD2-Cre mice injected with AAV-DIO-OxR2 displayed increased expression of OxR2 compared to mice injected with AAV-DIO-GFP as determined by ISH (two-tailed student’s t-test, n = 3 biologically independent mice, 2 slices per mouse, t(4)=4.417, p = 0.0069) (left). Representative image of GFP expression localized to GAD2 LHb neurons in GAD2-Cre mice injected with AAV-DIO-OxR2, scale bar = 25 μm (right). Experimental images were obtained from 7 biologically independent mice, 2 slices per mouse, with similar results obtained. c, Representative images of OxR2 expression in GAD2 LHb neurons infected with AAV-DIO-OxR2 or AAV-DIO-GFP, scale bar = 25 μm. *p < 0.05, **p < 0.01. Experimental images were taken from 7 biologically independent mice, 2 slices per mouse, with similar results obtained. All data are expressed as mean ± SEM.
