Extended Data Fig. 2: LHB GAD2 neuron fiber phohometry supporting data. | Nature Neuroscience

Extended Data Fig. 2: LHB GAD2 neuron fiber phohometry supporting data.

From: Orexin signaling in GABAergic lateral habenula neurons modulates aggressive behavior in male mice

Extended Data Fig. 2

a, AGG LHb GAD2 neuron activity was increased following bites on day 1 of RI (two-tailed paired t-test, n = 5 biologically independent mice, 2-5 bites per mouse, t(4)=4.008, p = 0.016). b, AGG LHb GAD2 neuron activity was reduced following a withdrawal from an aggressive encounter on day 1 of RI (two-tailed paired t-test, n = 5 biologically independent mice, 3-5 withdrawals per mouse, t(4)=3.982, p = 0.0164). c, AGG LHb GAD2 neuron activity did not differ before and after random times points on day 3 of RI (two-tailed paired t-test, n = 5 biologically independent mice, 5 time points per mouse, t(4)=0.493, p = 0.6475). d, NON LHb GAD2 neuron activity was not different before and after an approach on day 1 of RI (two-tailed paired t-test, n = 5 biologically independent mice, 3-5 approaches per mouse, t(4)=1.843, p = 0.1406). e, NON LHb GAD2 neuron activity was not different before and after a withdrawal from a non-aggressive social interaction on day 1 of RI (two-tailed paired t-test, n = 5 biologically independent mice, 3-5 withdrawals per mouse, t(4)-1.633, p = 0.1777). f, NON LHb GAD2 neuron activity did not differ before and after random time points on day 3 of RI (two-tailed paired t-test, n = 5 biologically independent mice, t(4)=1.721, p = 0.1634). g, GAD2-cre AGGs used for fiber photometry experiments displayed significantly higher aggression CPP scores than GAD2-cre NONs (two-tailed paired t-test, n = 5 biologically independent mice, t(4)=2.885, p = 0.0448). h, LHb GAD2 neurons peaks in the intruder paired context during the CPP preference test were positively correlated with CPP score (two-tailed student’s t-test,A n = 10 biologically independent mice, Pearson correlation coefficient = 0.761 m, R2 = 0.586, p = 0.0161). *p < 0.05, **p < 0.01. *p < 0.05. All data are expressed as mean ± SEM.

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