Extended Data Fig. 3: Diversity of astrocyte states in WT and 5xFAD mice.
From: Disease-associated astrocytes in Alzheimer’s disease and aging
a, 2-D Umap25 embedding of 7,345 single nuclei RNA profiles of astrocytes (as in Fig. 1d) from hippocampus of 4 WT (left) and 4 5xFAD (AD, right) 7-month old mice. Colored by cluster, all other cells in light yellow in the background. b, 2-D Umap25 embedding as in (a), colored by sample (left), or batch (right). c, Number of genes and transcripts across clusters. Violin plots showing the distribution of number of genes (top) and transcripts (unique UMIs, bottom) detected per cluster (n=8 animals, total of 7,345 cells). Cluster numbers as in (a). d, Diffusion maps28 of 7,345 single nuclei RNA profiles of astrocytes in the hippocampus of WT and 5xFAD mice, showing 2-D embedding of cells in combinations of the top four diffusion components (DC), colored by mouse strain, WT (blue) and 5xFAD (AD, red). e, Distribution of astrocyte states in WT and AD brains. Box plots showing the fraction of each astrocyte cluster (compared to total number of astrocyte cells, clusters as in Fig. 1d, n=8 animals, 10 samples), in WT and 5xFAD mice. Displaying the median (thick lines), 25% and 75% quantiles (box), and individual samples (dots).
