Extended Data Fig. 5: Shared and distinct transcriptional programs of DAAs and physiological Gfap-high astrocytes.
From: Disease-associated astrocytes in Alzheimer’s disease and aging
a, Differential expression across astrocyte states. Volcano plots showing differentially expressed genes in each pair of states (n=8 animals, 10 samples. y-axis: -log adjusted hypergeometric p-value, following FDR multiple hypothesis correction, x-axis: average log fold change). AD risk factor genes from GWAS marked in orange (as in Fig. 2a). b, DAAs, Gfap-high astrocytes, and cluster 3 astrocytes share multiple upregulated genes and pathways compared to the homeostatic Gfap-low astrocyte population, but also have distinct expression programs. Pathway (rows) enrichment for upregulated genes in cluster 4 (C4, DAAs, n=478 cells), cluster 6 (C6, Gfap-high, n=457), or cluster 3 (C3, intermediate state, n=1,666 cells), compared to Gfap-low astrocytes (n=1,594 cells). Enriched pathways30 (hypergeometric p-value with FDR<0.05. n=8 animals, 10 samples), colored by -log FDR values (as in Fig. 2b, with full list of pathway annotations and no scaling).
