Extended Data Fig. 3: p53 status determines MHC-I expression in medulloblastoma patient-derived xenografts and DIPG cells.
From: RETRACTED ARTICLE: Tumor necrosis factor overcomes immune evasion in p53-mutant medulloblastoma
(a–j) FACS histograms of HLA-I expression in human medulloblastoma PDXs expressing mutant p53 (upper panel, a-e) or wild-type p53 (lower panel, f-j. Isotype controls are shown in black and HLA-I staining in purple. e,j, Quantification of average mean fluorescence intensity (MFI) for triplicate samples of each human medulloblastoma PDX. Two-sided t-tests were used to evaluate the significance of the difference between HLA-I and isotype control staining for p53-mutant tumors (e, NS, not significant, P= 0.3793) and for p53-WT tumors (j, *P= 0.0112). k–n, FACS histograms of MHC-I expression in p53 wild-type (k, m) or p53 deficient (l and n) murine DIPG lines (k-l) and human DIPG lines (m,n). Isotype controls are shown in black and murine MHC-I and human HLA-I staining in purple. Quantification of the MFI values for three independent tumor samples are shown below each histogram; data points represent MFIs for individual tumor samples. p-values were determined by two-sided unpaired t-test and are indicated on each bar graph.
