Extended Data Fig. 5: Perturbation of Tap1 alters expression of cell surface MHC-I.
From: RETRACTED ARTICLE: Tumor necrosis factor overcomes immune evasion in p53-mutant medulloblastoma
a–d, MG tumor cells were transduced with control shRNA (shCtl) or shRNAs targeting Tap1 (shTap1#1, shTap1#2). Knockdown efficiency was determined by western blotting (a). Western blots are cropped at the molecular weights for Tap1 (68 kDa) and β-actin (42 kDa); original blots are available in Source Data. MHC-I expression was determined by FACS in control cells (shCtl, black) and Tap1 knockdown cells (shTap1, red) (b). Quantification of the mean fluorescence intensity (MFI) values for three independent tumors is shown below each histogram; data points represent MFIs for individual tumor samples. p-values, determined by two-sided unpaired t-test, are indicated on bar graph. c-d, Tap1 knockdown cells were transplanted into aB6 mice. Bioluminescence imaging of representative mice (c) and survival curves (n = 6) (d) are shown. p-values for the difference in survival between shTap1 and shCtl were determined using the two-sided log-rank (Mantel-Cox) test. *P = 0.0198 (shTap1#1) and *P= 0.0246 (shTap1#2). (e, f) MP tumor cells were transduced with empty vector (vect) or vectors encoding Erap1 or Tap1. Analysis of MHC-I expression by FACS in control cells (vect, black), Erap1-overexpressing cells (e) or Tap1-overexpressing cells (f) (blue histograms in e and f). Quantification of the MFI for three independent experiments is shown below the histogram; data points represent MFIs for individual tumor samples. The p-values were determined by two-sided unpaired t-test and are indicated on each bar graph.
