Extended Data Fig. 9: Representative gating strategy used for analysis of HLA A/B/C in human cells or MHC-I expression in murine cells.
From: RETRACTED ARTICLE: Tumor necrosis factor overcomes immune evasion in p53-mutant medulloblastoma
a–d, Gating strategy for unlabeled human tumor cells (data for RCMB18 are shown). (a) Dot plot representing FSC-A and SSC-A profile of the entire cell population, with box indicating intact cells (as opposed to debris and clumps) selected for analysis. (b) Dot plot showing the FSC-H and FSC-A profile of the population selected in (a), with a box indicating the single cell (as opposed to doublet) population selected for analysis. (c) Dot plot showing 7-AAD staining vs. FSC-A of the population in (b), with a box indicating the viable (7-AAD-negative) cell population selected for analysis. (d) Analysis of HLA-I staining of the cell population in (c). (e–i) Gating strategy for GFP-labeled murine (MG) tumor cells. (e) Dot plot representing FSC-A and SSC-A profile of the entire cell population, with box indicating intact cells (as opposed to debris and clumps) selected for analysis. (f) Dot plot showing the FSC-H and FSC-A profile of the population selected in (e), with a box indicating the single cell (as opposed to doublet) population selected for analysis. (g) Dot plot showing 7-AAD staining vs. FSC-A of the population in (f), with a box indicating the viable (7-AAD-negative) cell population selected for analysis. (h) Dot plot showing GFP expression vs. FSC-A in the population selected in (g), with a box indicating the tumor cells (GFP+) selected for analysis. (i) Analysis of MHC-I staining of the population in (h).
