Extended Data Fig. 5: Manipulations of CeAGA neurons modulated reflexive withdrawal threshold to von Frey filaments and activation of CeAGA neurons did not alter courtship behaviors.
From: General anesthetics activate a potent central pain-suppression circuit in the amygdala
a, b, Quantifications of the withdrawal threshold to von Frey filaments applied to the whisker pad in a, naïve (control, n = 9 animals (0.44 ± 0.0g5 (ipsi-off), 0.39 ± 0.05g (ipsi-on), 0.39 ± 0.06g (contra-off), 0.36 ± 0.06g (contra-on)), ChR2, n = 8 animals (0.34 ± 0.06g (ipsi-off), 0.95 ± 0.05g (ipsi-on), 0.52 ± 0.12g (contra-off), 0.93 ± 0.08g (contra-on)), eArch, n = 7 animals (0.21 ± 0.07g (ipsi-off), 0.06 ± 0.01g (ipsi-on), 0.35 ± 0.07g (contra-off), 0.22 ± 0.05g (contra-on)); two-way ANOVA; ****P < 0.0001, **P = 0.0042; F6,84 = 10.80) and b, IoN-CCI mice (control, n = 6 animals (0.28 ± 0.09g (off), 0.39 ± 0.14g (on)), ChR2, n = 6 animals (0.16 ± 0g (off), 0.87 ± 0.08g (on)); two-way ANOVA; **P = 0.0032; F1,20 = 10.54). c-d, Quantification of light-illumination induced changes in total syllable number of ultrasonic vocalizations (GFP: 23.25 ± 35.65; ChR2: 21.17 ± 44.17), or total duration of anogenital sniffing and mounting behavior (GFP: 16.25 ± 4.31 s; ChR2: 18.17 ± 4.53 s) in control CeAGA-GFP and CeAGA-ChR2 mice (with light - without light) during 2 min of social interactions (control, n = 4 animals, ChR2, n = 6 animals; unpaired t-test, two-tailed; P = 0.974, F5,3 = 2.303 (syllable number), P = 0.7791, F5,3 = 1.656 (anogenital sniffing)). Data are mean ± s.e.m.
