Extended Data Fig. 6: iMG exhibit lipid accumulation when co-cultured with post-axotomy neurons.
a, Composite images of DRG neuronal explants before (left) and after (right) axotomy. n = 4 biologically independent experiments, with similar results. Scale bar, 500 µm. b, mCherry (yellow) fluorescence in neurons co-cultured with WT (top), PLCG2 KO (middle), or TREM2 KO (bottom) iMG without axotomy shows no neuronal degeneration at 0, 9, and 15 hours. n = 4 DRG explants from independent animals per condition, with similar results. Scale bar, 50 µm. c, Timecourse of neuronal debris accumulation within iMG in indicated co-culture conditions, quantified as percentage of mCherry+ iMG. n = 4 DRG explants from independent animals per condition. Data are mean ± s.e.m.; two-way repeated measures ANOVA, post-hoc Dunnett’s test for multiple comparisons. d, BODIPY staining (green) of neutral lipids shows accumulation in iMG co-cultured with DRG neurons from 0 to 18 hours post-axotomy. n = 4 biologically independent experiments, with similar results. Scale bar, 50 µm.
