Extended Data Fig. 4: Postnatal neurovascular maturation in the cerebral cortex of 16p11.2df/+ and WT mice.
From: Vascular contributions to 16p11.2 deletion autism syndrome modeled in mice
a–c, Postnatal developmental profile of cerebral cortex endothelial networks in 16p11.2df/+ and WT males (cortical layers where most significant differences were found). d-i, Postnatal developmental profile of cerebral cortex endothelial networks in 16p11.2df/+ and WT females. j, Sample image of the computational approach used to delineate ROIs in the cortex to quantify neuronal density (see methods for details). k, Quantification of neuronal density in the parietal (that is, somatosensory) cortex of female mice following immunostaining for neuronal markers NeuN and TBR1. l, Vascular endothelial growth factor-A (VEGF-A) levels measured by E.L.I.S.A. in protein extracts from cerebral cortex micro-dissected at P14 or P50 in male and female mice. WT, Wild-Type. Data are mean ± s.e.m. in a-i and k, or mean with individual values in l (n = 3-6 animals per group). *P < 0.05 (two tailed Mann-Whitney test). #P < 0.05, ###P < 0.001 (2-way ANOVA and Sidak’s post-hoc test).
