Extended Data Fig. 7: CLADES 1.0 progresses over larval development. | Nature Neuroscience

Extended Data Fig. 7: CLADES 1.0 progresses over larval development.

From: A programmable sequence of reporters for lineage analysis

Extended Data Fig. 7

a, Progression of the CLADES 1.0 cascade over the course of larval development, as triggered by the ubiquitous U6-gRNA#1 trigger. Green, red and gray, immunohistochemistry for YFP, RFP and Dpn respectively. n=10 brains. Scale bars = 50 micrometers. b, Percentage of neuroblasts (n=10 brains, 30 neuroblasts each condition) expressing the different reporter combinations. c, Normalization of the data shown in (b) to the maximum percentage for each combination of reporters. Error bars and areas around the line plot represent a 95% confidence interval. n=10 brains, 30 neuroblasts each condition.

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