Extended Data Fig. 8: CLADES 2.0 reflects the birth-order of the embryonic lineage 5-6 in the VNC.
From: A programmable sequence of reporters for lineage analysis
a, Schematics representing the organization of the lineage 5-6 in the VNC, labeled with CLADES 2.0 and the lbe(k)-GAL4 driver. The position of the neuroblast near the ventral surface generates a gradient with early-born neurons (green) pushed away towards dorsal positions, late-born neurons (red) located near the ventral surface and mid-born neurons (yellow) occupying intermediate positions. b, Representative example of a lineage 5-6 clone labeled with CLADES 2.0+lbe(k)-GAL4. Maximum intensity projection of multiple confocal planes. Green, red immunohistochemistry for V5 (YFP) and RFP respectively. Scale bar = 5 μm. n=9 clones c, Average depth from the ventral surface for neurons expressing each combination of reporters, both for clones with green, yellow and red or only green and yellow neurons. n=126 neurons, 9 clones in total.
