Extended Data Fig. 2: Critical frequency for dendritic electrogenesis in L5 pyramidal subclasses.
From: Active dendritic currents gate descending cortical outputs in perception
a, Left, in vitro whole-cell recording from PT (Sim1-Cre+) neurons in S1. Middle, increase in the after-depolarizing potential (ADP) evoked by high-frequency trains of four APs – indicative of dendritic regenerative potentials7. The inset shows the magnified ADPs after the last AP. Right, the sizes of somatic ADP as a function of AP frequency, fitted with a sigmoidal curve. The critical frequency was estimated from the turning point of a sigmoidal fit. b, Same as a, for IT (Tlx3-Cre+) neurons in S1. c,d, Critical frequencies and the changes in ADP size of PT neurons (orange; n = 17 neurons) versus IT neurons (purple; n = 17 neurons) (P = 9.1 × 10−4 for critical frequency, Student’s t-test; P = 2.2 × 10−3 for the change in ADP size, Mann-Whitney U test).
