Extended Data Fig. 2: E2 regulatory element to drive expression of reporters.
From: Viral manipulation of functionally distinct interneurons in mice, non-human primates and humans
Adult mice were injected systemically with rAAV-E2-dTomato. a, b, Slice recording of the intrinsic properties of virally labeled neurons in S1 cortex and PFC. The left panels show plots of recorded cells with the indicated intrinsic properties. The blue dots represent cells with stereotypical fast-spiking properties. The right panels indicate the proportion of fast spiking cells recorded. c, Representative slice recording traces of cells indicated in (b). (d) Representative image of virally labeled chandelier cells. (e) Coronal and sagittal sections were analyzed with IHC for the viral reporter and PV, and the specificity to PV was reported across brain regions. (f) The native viral expression was analyzed from the indicated organs. Scale bars represent 50um (d), 100um (e) and 250um (f). On the graphs, dots represent individual measurements and the lines represent average + /- s.e.m. Values for specificity are listed in the supplementary table 2. See method section for details on the reproducibility of the representative images presented in panel d and f.
