Extended Data Fig. 5: Additional staining approaches for XNH imaging.
From: Dense neuronal reconstruction through X-ray holographic nano-tomography
a, Top: Photograph of fly brain with GABAergic nuclei labeled with APEX2 (arrows). Middle: XNH images (120 nm pixels, 15 µm thick minimum intensity projection) of the same fly brain after heavy metal staining, showing clusters of dark, APEX2 labeled GABAergic cell nuclei (arrows). Bottom: XNH virtual slice (120 nm thick) and output from an automated Random Forest image classifier trained to detect labeled cells (green). b, XNH data (105 nm voxels) of a Drosophila brain that did not undergo heavy metal staining. Even in unstained soft tissue, phase-contrast imaging provides enough signal that single neurons can still be resolved. FOV encompasses the optic lobe and half of the central brain. See Supplementary Video 6 and Methods.
