Extended Data Fig. 2: Brainwide extra-hippocampal input to vCA1 neurons that project to LH, BA, NAc, adBNST, LS and mPFC.
From: Circuit and molecular architecture of a ventral hippocampal network
a–d. Fraction of extrahippocampal input from nuclei in the (a) thalamus, (b) amygdala, (c) contralateral CA3, (d) midbrain/hindbrain and (e) basal forebrain/septum. See Methods for all abbreviations. n= 6 vCA1-mPFC, 8 vCA1-NAc, 9 vCA1-LH, 9 vCA1-BA, 4 vCA1-adBNST and 3 vCA1-LS mice . Error bars represent standard error of the mean. See Extended data table 2 for proportion counts (mean ± SEM) for all assayed input regions and all statistical values. f. Controls for non-specific rabies infection. No long-range inputs were labeled in mice injected with Cre-dependent rabies helper virus and EnvA-G deleted rabies mCherry virus in the vCA1 subregion of vHPC in the absence of AAV2retro Cre injection (n=2 mice, no long-range input cells detected). right, image of red channel from thalamus, basal forebrain and amygdala of control mice. g. Number of extrahippocampal inputs correlates with the number of starter cells in RV samples (Pearson’s r=0.4035, two-tailed, p=0.037). AA-anterior amygdala, AD-anterodorsal nucleus, AM-anteromedial nucleus, BLA-basolateral amygdala, BMA-basomedial amygdala, CeA-central amygdala, CL-central lateral nucleus of the thalamus, CLI-central linear nucleus raphe, CM-central medial nucleus of the thalamus, CoA-cortical amygdala, DR-dorsal raphe nucleus, DTN-dorsal tegmental nucleus, IAD-interanterodorsal nucleus, IF-interfascicular nucleus raphe, IMD-Intermediodorsal nucleus of the thalamus, IPN-interpeduncular nucleus, LA-lateral amygdala, LC-locus coeruleus, LDT-laterodorsal tegmental nucleus, LHA-lateral hypothalamic area, LP-lateral posterior nucleus of the thalamus, LS-lateral septum, MR-median raphe, MA-magnocellular nucleus, MD-intermediodorsal nucleus of the thalamus, mPOA-medial preoptic area, MS-medial septum, MY-medulla, NDB-diagonal band nucleus, NI-nucleus incertus, NLoT-nucleus of lateral olfactory tract, P-pons, PAA-piriform-amygdalar area, PAG-periaqueductal gray, PB-parabrachial nucleus, PCG-pontine central gray, PF-parafascicular nucleus, PPN-pedunculopontine nucleus, PR-perireunensis nucleus, PRNr-pontine reticular nucleus, PT-parataenial nucleus, PVH-paraventricular nucleus of hypothalamus, PVT, paraventricular nucleus of the thalamus, RE-nucleus of reuniens, RPO-nucleus raphe pontis, RT- reticular nucleus of the thalamus, SC- superior colliculus, SG-supragenual nucleus, SI- substantia innominate, SUT-supratrigeminal nucleus, TR-piriform transition area, VAL-ventral anterior-lateral complex of the thalamus, VII-facial motor nucleus, VM-ventral medial nucleus of the thalamus, VTA-ventral tegmental, Xi-xiphoid thalamic nucleus.
