Extended Data Fig. 3: Transcriptional profiling of vCA1 projection neurons.
From: Circuit and molecular architecture of a ventral hippocampal network
a. Volcano plots of differentially expressed genes for each set of pairwise comparisons. Red dots indicate differentially expressed genes that passed p<0.05 cutoff using Wald test, two-sided, and after correction for multiple comparisons with Benjamini Hochberg. All exact p-values provided in Extended Data Table 4. b. Normalized relative expression of the all 24 genes that passed the significance threshold for differential expression in pairwise comparisons . n=3 vCA1 to mPFC replicates, 2 vCA1 to LH replicates, 3 vCA1 to NAc replicates and 3 vCA1 to BA replicates. Error bars indicate SEM. c. RNAscope of CDR1 transcript in vCA1 neurons defined by their projection to the mPFC. Arrowheads indicate co-labeled neurons. Scale bar 40 μm. Right. Quantification of overlap in CTB labeled and CDR1 labeled cells, n=9 FOVs from 2 mice, error bars indicate SEM. d. Heatmap correlations of total log2 normalized read counts from individual sample sets used for profiling experiments. e. Principal components plots of 500 most variable genes in the dataset, showing first 5 PCs.
