Extended Data Fig. 4: Additional E06 analysis and OxPC neutralization.

a, Representative confocal microscopy images comparing Ctrl PBS injected spinal cord white matter and POVPC injected spinal cord white matter after 1, 3, or 7 days. Sections were labeled with DAPI, E06, Tuj1 and IBA1. Data for each time point was acquired from 2 to 3 separate experiments with 3 to 5 mice in each experiment. b, Representative widefield microscopy images of primary mouse neurons labeled with Tuj1 for tubulin β3 24 h after EtOH control, POVPC (25 µM), POVPC + IgM (10 µg/ml), or POVPC + E06 (10 µg/ml) treatments. DAPI was used to label nuclei. c, Bar graph comparing the fold change in the number of DAPI⁺ Tuj1⁺ primary mouse neurons 24 h after treatment, normalized to the EtOH control. Data acquired from 2 separate experiments each with 4 replicates. Significance indicated as *** p < 0.001, one-way ANOVA comparing the treatments against the EtOH control. d and g, Representative confocal images of the ventral spinal cord 3 days after ATP (30 mM) + IgM (500 µg/ml) or ATP (30 mM) + E06 (500 µg/ml) injection. Sections were labeled with DAPI, E06, and IBA1 in d, or with MBP and NF-H in g. e,f, Bar graphs comparing the percent E06⁺ deposition (e) or percent IBA1⁺ (f) found per field of view (FOV) in spinal cord of mice 3 days ATP + IgM or ATP + E06 injection. h, Representative particle analysis masks comparing the number of NF-H + axons with surface area smaller or larger than 30 µm2 in the two treatment groups. Data was acquired from 2 separate experiments with 5 mice per experiment. Significance indicated as * p < 0.05, ** p < 0.01, two-tailed, unpaired t-test. All data presented as mean values with error bars showing +/− SD.