Extended Data Fig. 5: Additional neuron microglia co-culture experiments.

a, Representative microscopy images of primary mouse neurons cultured with or without microglia (MG) and then treated with EtOH, POVPC (50 µM), or POVPC with CytoD for 24 h, labeled with DAPI, Tuj1, CD68, and E06. b, Representative images of mouse neurons treated with 0.015% EtOH control (top left), pre-treatment with 200 nM latrunculin A (LA) for 30 min followed by 0.015% EtOH (top middle) or 50 µM POVPC (top right); and neurons and microglia pre-treated with LA for 30 min followed by EtOH (bottom left), POVPC (bottom middle), or LA for 30 min followed by 50 µM POVPC treatment for 24 h (bottom right). c, Bar graph comparing the fold change in the number of DAPI⁺ Tuj1⁺ cells after treatments with or without POVPC, LA, and microglia. Data acquired from 2 separate experiments each with 4 replicates. Significance indicated as * p < 0.05, *** p < 0.001, one-way ANOVA comparing the treatments against the EtOH control. All data presented as mean values with error bars showing +/− SD.