Extended Data Fig. 3: PTB-ASO application into human organoid cultures leads to increase levels of TuJ1 protein with no alterations in SOX2 or caspase 3 markers.
From: Therapeutically viable generation of neurons with antisense oligonucleotide suppression of PTB
a, Representative images of 5 months old human organoid taken 1-month post-PTB-ASO2 or control treatment. Images show a view of the axonal marker (green) TUJ1 (visualized by immunofluorescence); DAPI staining for DNA; experiment was reproduced three times, independently, with similar results. b, Total TuJ1 area in PTB-ASO treated organoids compared to control ASO. Data are presented as fold change mean +/− SEM (control mean: 1 +/− 0.21; PTB-ASO mean: 1.73 +/− 0.33; n = 3 biological repeats). c, Representative images and from 5 months old human organoid taken 1-month post-PTB-ASO2 application to the culture medium. Images show a view of (green) SOX2 (visualized by immunofluorescence); experiment was reproduced three times, independently, with similar results. d, Quantification of the total SOX2 positive cells per organoid area treated with PTB-ASO compared to ASO control. Data are presented as mean +/− s.e.m. (control mean: 436.7 +/− 17.82; PTB-ASO mean: 406.3 +/− 19.89; n = 3 biological repeats). e, Total caspase 3 (CC3) protein area in PTB-ASO treated organoids compared to control ASO. Data are presented as fold change mean +/− s.e.m. (control mean: 1 +/− 0.21; PTB-ASO mean: 0.79 +/− 0.19; n = 4 biological repeats).
