Extended Data Fig. 3: Effects of ketamine on pre-and post-synaptic function at 7 days after treatment.

a, Saline or ketamine was administered to C57BL/6J mice. The mice were sacrificed 7 days later, and slices were prepared for recordings. I-O curves were measured during baseline recording in the SC-CA1 synapses. I-O curves of Sal and Ket groups were from baseline recording in the Sal-Ket group of Fig. 3b and Ket-Ket group of Fig. 3c, respectively. The slope of I-O curves in the previous ketamine-treated group was not significantly different compared to the saline-treated group (two-sided unpaired t-test, t₍₁₇₎ = 1.448, P = 0.1659, Sal, Ket: n = 9, 10 slices). b, PPRs were measured before and after ketamine perfusion onto hippocampal slices of mice given either saline or ketamine 7 days before the slice preparation. PPRs were not significantly changed by either previous ketamine injection or subsequent ketamine perfusion (two-way ANOVA with Tukey’s multiple comparisons, all P-values > 0.05, Sal-Ket, Ket-Ket: n = 11, 10 slices). Representative traces are from data recorded at 30 msec interstimulus interval in the respective treatment groups. Graphs represent mean ± S.E.M., N.S.: not significant, Sal: saline, Ket: ketamine. Sal+Ket: ketamine perfusion onto hippocampal slices from saline-injected mice, Ket+Ket: ketamine perfusion onto hippocampal slices from ketamine-injected mice. For detailed statistical information, see Supplementary Table 1.