Extended Data Fig. 2: Ketamine rapidly increases BDNF protein levels in the hippocampus of Mecp2 S421A KI mice. | Nature Neuroscience

Extended Data Fig. 2: Ketamine rapidly increases BDNF protein levels in the hippocampus of Mecp2 S421A KI mice.

From: Sustained effects of rapidly acting antidepressants require BDNF-dependent MeCP2 phosphorylation

Extended Data Fig. 2

BDNF protein levels were measured 30 min after ketamine treatment in Mecp2 KI and CTL mice by Western blot analysis. BDNF levels were significantly increased by ketamine treatment in both the CTL and KI mice (two-way ANOVA with Tukey’s multiple comparisons, Genotype x Drug: F(1, 21) = 0.0157, P = 0.9016, Genotype: F(1, 21) = 0.0115, P = 0.9158, Drug: F(1, 21) = 31.93, P < 0.0001, CTL-Sal, CTL-Ket, KI-Sal, KI-Ket: n = 7, 7, 6, 5 mice). In Western blot analysis for pMeCP2, membrane above about 70 KDa was cropped and used for immunoblotting. The graph represents mean ± S.E.M., **, P < 0.01, Sal: saline, Ket: ketamine, CTL: littermate control. For detailed statistical information, see Supplementary Table 1.

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