Extended Data Fig. 1: TNSST neuronal activity is increased with palatable diets.
From: A neural circuit for excessive feeding driven by environmental context in mice
a, Representative images of FISH for VGAT (red) and cFos (green) mRNA and quantification of c-Fos-positive LHVGAT neurons in mice after fasting (7 sections from n = 3), or consuming chow (8 sections from n = 4) or HFD (8 sections from n = 4), or chocolate pellets (14 sections from n = 3) for 1 hour post-fasting (one-way ANOVA with Tukey’s multiple comparison test F(3, 33) = 0.7216; p(fasted vs chow)= 0.9183; p(fasted vs high fat)= 0.6731; p(fasted vs choc)= 0.9787). b, Representative images of FISH of VGAT (red) and cFos (green) mRNA and quantification of c-Fos-positive ZIVGAT neurons after fasting (7 sections from n = 3), or consuming chow (8 sections from n = 4 mice) or HFD (7 sections from n = 4) or chocolate pellets (14 sections from n = 3) for 1 hour post-fasting (F(3, 32) = 1.140; p(fasted vs chow)= 0.2928; p(fasted vs high fat)= 0.9994; p(fasted vs choc)= 0.8855). Scale bars (a and b), 200 m. c, Quantification of cFos-positive TNSST neurons rostro-caudally in mice after fasting (18 sections from n = 4), or consuming normal chow (20 sections from n = 5) or HFD (19 sections from n = 6) for 1 hour post-fasting (Anterior: F(2, 12) = 11.86, p(fasted vs chow)= 0.2949, p(fasted vs high fat)= 0.018, p(chow vs high fat)= 0.0011; Medial: F(2,25)= 26.33, p(fasted vs chow)= 0.1555, p(fasted vs high fat)= 0.0001, p(chow vs high fat)<0.0001; Posterior: F(2,11)=29.49, p(fasted vs chow)= 0.1089, p(fasted vs high fat)= 0.0013, p(chow vs high fat)<0.0001, **p < 0.01). d, Quantification of Ghsr+ and Ghsr- c-Fos+ TNSST neurons along rostro-caudal axis after consuming HFD for 1 hour post- fasting (Ghsr + : F(2,10)=2.101; p = 0.1730; Ghsr-: F(2,10)=2.101; p = 0.1730). e, Quantification of cFos+ TNSST neurons using immunohistochemical staining, with representative coronal sections after fasting (17 sections from n = 3), or consuming normal chow (17 sections from n = 3) or HFD (18 sections from n = 3) for 2 hour post-fasting (F(2,49)=37.57; p(fasted vs chow)= 0.9273; p(fasted vs high fat)<0.0001; p(chow vs high fat)<0.0001, ***p < 0.001). One-way ANOVA with Tukey’s multiple comparison test was used for all panels in this figure. All data in figure are presented as mean values±SEM.
