Extended Data Fig. 2: Further characterisation of ^TNSST neuronal response to palatable diets.

a, Experimental design for fibre photometry experiment. b and c, Population activity of ^TNSST neurons (mean ± SEM) while approaching chocolate pellets (b) (two-sided paired t-test p = 0.0231), and while consuming chocolate pellets (c) (two-sided paired t-test p = 0.0417). d, Experimental design showing microendoscopic imaging in freely behaving mice in an arena with high fat corner (HFC) and chocolate corner (CC). e, Venn diagram showing overlap between CP-responsive and HFD-responsive ^TNSST neurons. f, Traces of ^TNSST activity (mean ± SEM) when entering the HFC or CC corner in the arena. g, Experimental strategy to identify proportion of ^TNSST neurons responding to both HFD and CP. The schematic shows that ^TNSST neurons were TRAPed in response to HFD (similarly, the CP-responsive ^TNSST neurons were TRAPed by changing HFD with CP). h, Images showing ^TNSST neurons responding to HFD (#), CP(*) or to both (white arrows) when HFD was paired with 4-OHT administration for TRAP. i, Images showing ^TNSST neurons responding to HFD (*), CP(#) or to both (white arrows) when CP was paired with 4-OHT administration for TRAP. # = YFP + Tdtomato + , * = YFP + c-Fos + , white arrow = YFP + Tdtomato+c-Fos + . Scale bar, 200 µm. j, Illustration showing how above strategy label putative neurons that respond to both HFD and CP. k, Pie graphs showing percentage of ^TNSST neurons responding to HFD, CP or to both quantified from (h) and (i) n = 3 mice for both pie graphs. All data in figure are presented as mean values±SEM.