Extended Data Fig. 7: There are few sex-specific differences in astrocytic gene expression during inflammation.
From: Neuroinflammatory astrocyte subtypes in the mouse brain
a, FPKM scatter plot showing all genes expressed >0.5 FPKM in astrocytes purified from male and female saline-injected Aldh1l1eGFP mice based on bulk sequencing. Genes that are differentially expressed with padj < 0.05 are highlighted in purple. Circled are the highest expressed genes according to FPKM specific to males and females, Eif2s3y and Xist, respectively. b, Bar graphs showing mean FPKM values of genes differentially expressed (* = padj<0.05, DESeq2) between male (n = 10) and female mice (n = 9). Circles are single animals, error bars are SEM. c, t-SNE feature plot highlighting female-specific gene Xist and male-specific gene Eif2s3y. d, Heatmap showing z-normalized FPKM levels of sex-specific LPS-induced genes. e, Scatter plot comparing log2 fold inductions due to LPS compared to saline-injected mice based on bulk sequencing. Genes included are >10 FPKM and padj<0.05 in either males or females as a consequence of LPS injection. f, Bar graphs showing mean FPKM values of example genes only induced in female mice (* = padj < 0.05, # = NS compared to male saline, DESeq2) Far1, Cdh19 and Tsc22d3. Circles are single animals, error bars are SEM.
