Extended Data Fig. 1: FACS strategy to purify GFP + astrocytes from Aldh1l1eGFP mice, initial clustering and cell type identification.
From: Neuroinflammatory astrocyte subtypes in the mouse brain
a, A single cell suspension of mouse cortex was prepared (see Methods) and cells from two hemispheres were recovered in 500 µL 1%BSA containing PBS. Cells were run on a Sony SH800Z with a 100 μm nozzle at 4 °C. Gating was set to capture single cells that were DAPI-negative (alive) and GFP-positive (astrocytes). 100.000 GFP + astrocytes were captured per mouse. Channels were: DAPI (405 nm) and GFP (488 nm). Abbreviations: SSC, side scatter; FSC, forward scatter, a, area; w, width. b,c, Initial clustering of all 91110 cells across all 12 animals identifies presence and percentage of non-astrocytic cells as defined by cell type marker genes in (d). d, Heatmap of normalized cell type marker genes across the identified cell type clusters.
