Extended Data Fig. 2: Category learning in a head-fixed operant conditioning setup.
From: Mouse visual cortex areas represent perceptual and semantic features of learned visual categories
a, Timeline showing training stages for head-fixed category learning (Methods). b, Trial sequence. c, Mean (±s.e.m.; n=8 mice) learning curve for head-fixed category learning. Gray represents individual animals. Category training stages are marked by Latin numerals. Insets show example stimulus spaces, with stimuli that were included at each stage in full contrast and not-yet-introduced stimuli in gray (stages II-IV are not shown). d, Mean (±s.e.m.; n=8 mice) response time of the first lick after stimulus onset, as a function of the stimulus’ distance from the category boundary. e, Fraction of left choices (mean ±s.e.m.; n=8 mice), as a function of the stimulus’ distance from the category boundary. Gray represents individual mice, averaged across all training sessions of stage VI. f, As in (e), but for mice performing the task at different stimulus positions (n=5). Colors indicate the position of the monitor at which mice performed the task (default position 26°). g, Example images from eye tracking cameras. Red dots show automated annotations made using DeepLabCut109,110. h, Horizontal normalized pupil position (Methods) during stimulus presentation, for stimulus positions −26° (monitor shifted) and 26° (default position). Gray represents individual mice, bars show mean ±s.e.m. (one-sided WMPSR test, left eye: W=2, P=0.094; right eye: W=2, P=0.094; n=5 mice). i, as (h), for pupil diameter (one-sided WMPSR test, left eye: W=0, P=0.031; right eye: W=15, P=0.031; n=5 mice). Note that, as in humans116, the ipsilateral pupil contraction (that is, the pupil reflex on the side where the monitor is positioned) is stronger than the contralateral contraction. All panels: NS (not significant) P>0.05; * P<0.05.
