Extended Data Fig. 6: Long-term GSK treatment of fully immersed CO slices in media no longer prevents EIF2α phosphorylation. | Nature Neuroscience

Extended Data Fig. 6: Long-term GSK treatment of fully immersed CO slices in media no longer prevents EIF2α phosphorylation.

From: Human ALS/FTD brain organoid slice cultures display distinct early astrocyte and targetable neuronal pathology

Extended Data Fig. 6

a and b, Western blots (WB) displaying p-EIF2α, EIF2α, β-actin levels in samples of H-L1 control and C9-L1 CO slices at 240 DIV (a) and ISO-L2 and C9-L2 CO slices at 200 DIV (b) immersed in media with or without 14-day treatment with GSK. Quantification of p-eIF2α and eIF2α protein levels as indication of UPR activation for H-L1/C9-L1 CO slice samples (a) and for the ISO-L2/C9-L2 CO slice samples (b). Data represent mean ± s.e.m. for ratios of band densities; n = 3 independent control CO slices and independent untreated/treated C9 CO slice pairs; Two-tailed unpaired t-test for the GSK effect. See Supplementary Table 3 for detailed statistics and Source Data for unprocessed WB images.

Source data

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