Extended Data Fig. 4: Clustering of human brain cells according to single nuclear RNA sequencing. | Nature Neuroscience

Extended Data Fig. 4: Clustering of human brain cells according to single nuclear RNA sequencing.

From: The SARS-CoV-2 main protease Mpro causes microvascular brain pathology by cleaving NEMO in brain endothelial cells

Extended Data Fig. 4

a, UMAP plot of 35,289 human brain cells. Clusters were annotated according to marker genes for the specific cell types. The dataset has been published (Lake, B.B., et al. Integrative single-cell analysis of transcriptional and epigenetic states in the human adult brain. Nat Biotechnol 36, 70-80 (2018)). b, Dot plots of marker genes used for annotation. Gene expression for all 30 clusters is shown as dot plot with the color representing mean gene expression and the diameters showing the percentage of positive cells in the clusters. Ex, excitatory neuronal subtypes; In, inhibitory neuronal subtypes. c, The SARS-CoV-2 S gene encoding the spike protein was found in vascular cells in the cortex of a COVID-19 patient (exemplary for at least 3 vessels) but not in an uninfected control subject by in situ hybridization. Scale bar, 50 µm.

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