Extended Data Fig. 7: Reduced Aβ deposition inside grafts of microglia-depleted Cx3cr1+/−/5xFAD mice.
From: Microglia contribute to the propagation of Aβ into unaffected brain tissue
(a) Experimental setup for the elimination of microglia and transplantation procedure in Cx3cr1+/−/5xFAD mice. (b) Fluorescence microscopy of Cx3cr1+/−/5xFAD cortex treated with vehicle (left) or CSF-1R inhibitor (right). Scale bar, 50 μm. (c) Quantification shows mean (± s.e.m.) of cortical microglia density after treatment. Each symbol represents one mouse of n = 6 mice per group. Significant differences were determined by the two-tailed Mann–Whitney test (**P = 0.0022). (d-e) Fluorescence microscopy of a graft in Cx3cr1+/−/5xFAD mice treated for 30 days with (d) CSF-1R vehicle or (e) CSF-1R inhibitor and immunostained for Aβ (6E10, red; white arrowheads indicate Aβ), and DAPI (blue). Scale bar, 50 μm. (f and g) Graphs show mean (± s.e.m.) of (f) microglia density and (g) percentage of Aβ inside the graft in the two groups of mice. Each symbol represents one graft from n = 8 mice per group. Significant differences were determined by the two-tailed Mann–Whitney test (****P < 0.0001; **P = 0.0031).
