Extended Data Fig. 8: Astrocytes accumulate in grafts of Irf8−/−/Cx3cr1+/−/5xFAD and microglia depleted Cx3cr1+/−/5xFAD mice.
From: Microglia contribute to the propagation of Aβ into unaffected brain tissue
(a,c) Confocal image of a graft in (a) Irf8+/−/Cx3cr1+/−/5xFAD (top), Irf8−/−/Cx3cr1+/−/5xFAD (bottom) and (c) Cx3cr1+/−/5xFAD mouse treated with vehicle (top) or with CSF-1R inhibitor (bottom) immunostained with an antibody against GFAP (red) and DAPI (blue). Scale bar, 50 μm. (b,d) Graphs show mean density (± s.e.m.) of GFAP-positive astrocytes in (b) Irf8/Cx3cr1+/−/5xFAD and in (d) microglia depleted mice. Each symbol represents one graft from n = 4 mice per genotype. Significant differences were determined by the two-tailed Mann–Whitney test.
