Extended Data Fig. 9: Microglia migrate and transport Aβ from the old Cx3cr1+/−/5xFAD to the young WT brain slices in the co-culture model.
From: Microglia contribute to the propagation of Aβ into unaffected brain tissue
(a-d) Confocal images of co-cultures of organotypic brain slices from aged Cx3cr1+/−/5xFAD and postnatal WT mice. Slices were immunolabelled with GFP (green), Thiazine Red (red) and Hoechst (blue). (a,c) Overview images of two representative co-cultures. Boxed regions in (a, c) are shown with a higher magnification in (b, d) and depict migrating 5xFAD microglia (GFP+) carrying Aβ (Τhiazine Red+) from the aged AD tissue towards the young WT tissue. Scale bars, 200 µm (a, c), 50 µm (b, d). n = 9 co-cultured brain slices from n = 2 mice per genotype.
