Fig. 2: Decreased Aβ deposition inside grafts in Irf8−/−/5xFAD recipient mice.
From: Microglia contribute to the propagation of Aβ into unaffected brain tissue
a, Representative confocal images (left) and corresponding Imaris-3D reconstruction (right) of microglia in the cortex of Irf8+/− and Irf8−/− mice. Scale bars, 15 μm in the left image and 10 μm in the 3D reconstruction on the right. For 3D reconstruction, two cells were considered in n = 2 mice per group. b–e, In vivo two-photon analysis of microglia process motility between 0 and 30 min after laser lesion ablation in Irf8+/+, Irf8+/− and Irf8−/− mice. Scale bar, 20 μm. Quantification of percentage of laser-responding cells (c), mean distance traveled (d) and speed of microglia processes (e) based on their distance from the injury site. Data are presented as mean (± s.e.m.). Significant differences were determined using two-way ANOVA followed by Sidak’s multiple comparison test (in c, ****P < 0.0001 and *P = 0.030), Tukey’s multiple comparison test F6,46 = 0.8661 (in d, range 31–60 μm: *P = 0.044 Irf8+/+ versus Irf8−/− and **P = 0.008 Irf8+/− versus Irf8−/−; range 91–120 μm: P = 0.054 Irf8+/+ versus Irf8−/− and *P = 0.0199 Irf8+/− versus Irf8−/−) and Tukey’s multiple comparison test F6,46 = 0.9551 (in e, range 31–60 μm: *P = 0.0484 Irf8+/+ versus Irf8−/− and **P = 0.0039 Irf8+/− versus Irf8−/−; range 91–120 μm: P = 0.0584 Irf8+/+ versus Irf8−/− and *P = 0.0235 Irf8+/− versus Irf8−/−). Data are from n = 4 Irf8+/+, n = 5 Irf8+/− and n = 6 Irf8−/− mice. f, Grafts in Irf8+/−/5xFAD and Irf8−/−/5xFAD recipient mice immunostained for Aβ (6E10, red; white arrowheads indicate Aβ depositions) and DAPI (blue). Scale bar, 100 μm. g,h, Graphs show mean (± s.e.m.) microglia density (g) and percentage of Aβ inside the grafts (h) in the two groups of mice. Each symbol represents one graft from n = 7 Irf8+/−/5xFAD and n = 6 Irf8−/−/5xFAD mice. Significant differences were determined using the two-tailed Mann–Whitney test (****P < 0.0001 in g and ***P = 0.001 in h).
