Fig. 3: In vivo two-photon imaging of Aβ-containing microglia movement after laser lesion.
From: Microglia contribute to the propagation of Aβ into unaffected brain tissue
a,b, In vivo two-photon imaging of Aβ-containing microglial responses at different time points after laser-induced tissue injury in Irf8+/+/Cx3cr1+/−/5xFAD mice (a) and Irf8−/−/Cx3cr1+/−/5xFAD mice (b). The top panels show merged images of GFP (green) and Thiazine Red (red) signal; the bottom panel shows only the Thiazine Red signal; asterisks indicate laser ablations; white arrowheads depict internalized Aβ moving with time; and yellow arrowheads indicate amyloid material associated with small microglia debris or no microglia association at all (shown only in the red channel). Scale bars, 50 μm. c–e, Graphs show mean (± s.e.m.) of Aβ accumulation at the lesion site 24 h post lesion (p.l.) in both groups of mice (c), relative increment of Aβ at the lesion site at 0, 6 and 24 h p.l. (d), and microglia accumulation at 0, 6 and 24 h p.l (e). Each symbol represents one laser lesion from n = 6 Irf8+/+/Cx3cr1+/−/5xFAD mice and n = 5 Irf8−/−/Cx3cr1+/−/5xFAD mice. Significant differences were determined using either the two-tailed Mann–Whitney test (**P = 0.0095 in c) or two-way ANOVA with Sidak’s multiple comparison test (*P = 0.0436 and **P = 0.0068 in d and *P = 0.0282 in e). NS, not significant.
