Extended Data Fig. 1: Aβ deposits in WT grafts.
From: Microglia contribute to the propagation of Aβ into unaffected brain tissue
(a,b) Fluorescence microscopy of grafts in Thy1-GFP/5xFAD recipient mice at (a) 4 weeks p.i. and (b) 16 weeks p.i. immunostained for Aβ (6E10, red and white; yellow arrowheads indicate the amyloid deposition) and DAPI (blue). Scale bar, 50 μm in the overview (a) and (b), 20 μm in the higher magnification (a), 25 μm in the higher magnification (b). n = 3 mice per incubation time. (c) Histogram showing the percentage of Aβ accumulation inside the grafts of Cx3cr1+/−/5xFAD recipient mice at 2, 4 and 16 weeks p.i.. Each symbol represents one graft from n = 4 (2 weeks p.i.), n = 9 (4 weeks p.i.) and n = 8 (16 weeks p.i.) mice. Data are presented as mean (± s.e.m.). Significant differences were determined by one-way ANOVA followed by Tukey’s multiple comparison test, F(2, 28) = 4.412 (*P = 0.0175). (d-f) Representative confocal microscopy showing (d) Aβ localization (6E10, red) in the graft and (e) high magnification acquisition (f) and Imaris-3D reconstruction of microglia (GFP, green) containing Aβ (red). Scale bars, 50 μm in (d), 10 μm in (e) and (f). n = 3 mice.
