Extended Data Fig. 2: Cellular composition of cortical WT grafts.
From: Microglia contribute to the propagation of Aβ into unaffected brain tissue
(a) Immunoblot analysis of cortical cell suspension that were used for grafting experiments. For the blot in the left panel, every protein was detected on separate membranes and only the loading control from the GFAP gel is shown. For uncropped gels with corresponding loading controls see Source Data Extended Data Fig. 2. n = 3 independent cell suspensions. (b) Representative confocal image of a graft in Thy1-GFP/5xFAD recipient mouse immunostained with NeuN (red) and DAPI (blue). n = 1 mouse. (c) Representative microscopy of a graft at 16 weeks p.i. of a Cx3cr1+/−/5xFAD recipient mouse immunostained with NeuN (red) and DAPI (blue). n = 1 mouse. (d) Confocal image of a graft in Cx3cr1+/−/5xFAD recipient mouse immunostained with Iba1 (red). n = 3 mice. (e) Confocal image of a graft in Hexb-tom/5xFAD recipient mouse transplanted with neurons from Cx3cr1-GFP embryos and counterstained with DAPI (blue). Scale bars 100 μm in (b), (c) and (e), 50 μm in (d). n = 4 mice.
