Extended Data Fig. 6: Quantification of Aβ-plaques in Irf8−/−/Cx3cr1+/−/5xFAD mice.
From: Microglia contribute to the propagation of Aβ into unaffected brain tissue
(a,c) Fluorescence microscopy of Irf8+/+/Cx3cr1+/−/5xFAD (left), Irf8+/−/Cx3cr1+/−/5xFAD (middle) and Irf8−/−/Cx3cr1+/−/5xFAD (right) cortices (a) and hippocampi (c) stained with Thiazine Red (red) and DAPI (blue). Scale bar, 100 μm in (a); 300 μm in (c). (b,d) Graphs show the mean (± s.e.m.) of Thiazine Red positive Aβ plaques in the three groups of mice in the cortex (b) and hippocampus (d). Each symbol represents one mouse from n = 5 mice per group. Significant differences were determined by one-way ANOVA followed by Tukey’s multiple comparison test. (e) Confocal microscopy of Irf8+/+/Cx3cr1+/−/5xFAD (left), Irf8+/−/Cx3cr1+/−/5xFAD (middle) and Irf8−/−/Cx3cr1+/−/5xFAD (right) cortices stained with Thiazine Red (red) and DAPI (blue). Scale bar, 30 μm; inset, 10 μm. (f) Graph shows the mean number (± s.e.m.) of microglia per plaque in the three groups. Each symbol represents one mouse from n = 5 mice per genotype. Significant differences were determined by Kruskal-Wallis test followed by Dunn’s multiple comparisons test, (*P = 0.014; *P = 0.04). (g) Confocal microscopy of Irf8+/+/Cx3cr1+/−/5xFAD (left), Irf8+/−/Cx3cr1+/−/5xFAD (middle) and Irf8−/−/Cx3cr1+/−/5xFAD (right) cortices immunolabeled with 6E10 antibody (red) and DAPI (blue). Scale bar, 10 μm. (h) Graph shows the mean (± s.e.m.) of Aβ internalized by microglia in the three groups of mice. Each symbol represents one mouse from n = 5 mice per group. Significant differences were determined by one-way ANOVA followed by Tukey’s multiple comparison test, F(2, 12) = 21.8 (**P = 0.0022; ****P < 0.0001).
