Extended Data Fig. 3: Cck-labeled neuropod cells express SGLT1 in the small intestine, related to Fig. 3.

a, Cck-labeled neuropod cells express SGLT1 and T1R3. b, Immunofluorescent image of small intestine tissue stained with SGLT1 (yellow). Most small intestinal epithelial cells, that is absorptive enterocytes and Cck-labeled neuropod cells (green), express SGLT1. c, Immunofluorescent image of proximal colonic tissue stained with SGLT1 (yellow). Minimal SGLT1 staining was observed in the colon. d, Fluorescent in situ hybridization (FISH) images of Cck-labeled neuropod cells in duodenal tissue. Top row–cell with expression of Cck, Tas1r3, and Slc5a1. Bottom row–cell with expression of Cck and Slc5a1, but not Tas1r3. e, Quantification of FISH results. In accordance with the single-cell qPCR results (Figs. 3b,c), 71.3 ± 0.04% of CCK+ cells only expressed transcripts for Slc5a1 while 28.7 ± 0.04% expressed transcripts for both Slc5a1 and Tas1r3 (N = 3 mice, n = 50 cells/mouse). Data are presented as mean values. Error bars = S.E.M. f, Normalized maximum vagal firing rate to baseline (PBS) and sucrose [300 mM] with and without SGLT2 inhibitor dapagliflozin [3 nM]. SGLT2 inhibition did not affect vagal firing in response to sucrose (N = 3 mice per group; *p = 0.0405 by ANOVA with post hoc Tukey’s HSD test). Data are presented as mean values. Error bars = S.E.M. g, Fold-change and p-values for genes shown in single cell qPCR heat map in Fig. 3b (N = 3 mice; n = 132 CckGFP + cells, n = 66 CckGFP- cells). h-i, Heat map of gene expression in CckGFP cells by single cell qRT-PCR. h, Genes significantly different between CckGFP cells positive and negative for Slc5a1 (SGLT1) (N = 3 mice, n = 132 CckGFP cells, 104/132 Slc5a1+). i, Genes significantly different between CckGFP cells positive and negative for Tas1r3 (T1R3) (N = 3 mice, n = 132 CckGFP cells, 31/132 Tas1r3+).