Extended Data Fig. 3: Realigning the data relative to heartbeat-induced movement artifact.

Realigning the data relative to heartbeat-induced movement artifact. a. Illustration of evidence of tissue movement relative to the electrode recordings in the LFP (shown in red-blue color scale with the range in µvolts shown in b). This is quantified by manually tracing these ‘band shifts’ using the Blender program, followed by detection of these movements in the LFP and tracking of these movements across channels (white line, second to rightmost plot). b. LFP before (left) and after (right) adjusting for movement effects. c. High frequency (action potential) frequency signal before (left) and after (right) adjusting for movement effects. d. Top row: Kilosort 3.0 registration and alignment alone could not compensate for the drift evident in the detected spike waveforms (left) and the estimated drift spanned hundreds of microns (right). Bottom row: manual alignment (a-b) followed by Kilosort 3.0 sorting resulted in improved spike alignment through time (left) and reduced drift (right).