Extended Data Fig. 1: Sampling times for each cue in Object Location Memory (OLM), ‘Where’, and ‘What’ tasks across age and sex.
(a) OLM (5-min training session, tested 24 hours later): Sampling times of displaced (Novel) vs the stationary (Familiar) objects were compared for male and female mice of prepubescent (Prepub) and adult ages. Prepubescent females and adult males preferentially sampled the displaced object (***P = 0.0001, ***P = 0.0006, respectively; 2-tailed paired t-test), whereas non-proestrus adult females and prepubescent males did not (n.s. P = 0.21, P = 0.73, respectively; N = 7-18/group). (b) OLM (5- or 10-min training, tested 24-hours later): Adult females trained for 5 minutes during proestrus stage preferentially sampled the displaced object over the stationary object (**P = 0.004). Non-proestrus adult females and prepubescent males were trained for 10 minutes. Non-proestrus females preferred the displaced object (**P = 0.01), but the prepubescent males did not (n.s. P = 0.46; N = 6-9/group). (c) OLM (5-min training, 15-min delay): Adult females did not spend more time with the moved object (P = 0.99), whereas Prepub males preferred the moved object (***P = 0.0002; N = 8/group). (d) Left. Schematic for episodic ‘Where’ task with four odors (see Methods). Right. Sampling times of odors A-D for each group during the 5-minute training trial (One-way ANOVA: P > 0.05 within all age groups). (e) Sampling times for the ‘switched’ pair (Novel) vs the stationary pair. Prepubescent females and adult males sampled the ‘switched’ pair more than the stationary pair (2-tailed paired t-test: Prepub female **P = 0.008, Adult male ****P = 0.00004). Prepubescent male and adult females showed no preference (P = 0.65, P = 0.97, respectfully; N = 8-11). (f) Left. Schematic of the ‘What’ task (see Methods). Right. Sampling times for each odor (One-way ANOVA: P > 0.05 within all age groups; N = 8-9/group). (g) Sampling times for novel odor vs mean of the three familiar odors (2-tailed paired t-test: ***P < 0.001, **P < 0.01; N = 8-9/group). Data are represented as mean ± SEM. Detailed statistics are found in Supplementary Table 1.
