Extended Data Fig. 5: ROCK inhibitor treatment further increases myelin mistargeting by surviving oligodendrocytes in the Tg(mbp:TRPV1-tagRFPt) zebrafish model. | Nature Neuroscience

Extended Data Fig. 5: ROCK inhibitor treatment further increases myelin mistargeting by surviving oligodendrocytes in the Tg(mbp:TRPV1-tagRFPt) zebrafish model.

From: New oligodendrocytes exhibit more abundant and accurate myelin regeneration than those that survive demyelination

Extended Data Fig. 5

(a) Confocal images of single oligodendrocytes which have not undergone demyelination treated with DMSO (control) or Y27632 ROCK inhibitor and imaged at 4dpf. Scale bar, 20 µm. (b) Confocal images of single surviving oligodendrocytes followed over time from prior to demyelination (csn treatment) at 4dpf through to 3dpt. Following demyelination oligodendrocytes were treated with either DMSO (control) or Y27632 ROCK inhibitor. Scale bars, 20 µm. (c) Quantification of the number of sheaths produced in oligodendrocytes which have not been demyelinated in control (mean = 16.08 ± 5.73 SD) and Y27632 ROCK inhibitor (mean = 19.35 ± 4.08 SD) treated zebrafish, p = 0.0386. Unpaired two-tailed t-test. N = 24 oligodendrocytes from 24 zebrafish (control), N = 20 oligodendrocytes from 20 zebrafish (Y27632). Data are presented as mean ± SD. (d) Quantification of the average sheath length (µm) produced in oligodendrocytes which have not been demyelinated in control (mean = 28.62 ± 7.18 SD) and Y27632 ROCK inhibitor (mean = 27.26 ± 6.91 SD) treated zebrafish, p = 0.5308. Unpaired two-tailed t-test. N = 24 oligodendrocytes from 24 zebrafish (control), N = 20 oligodendrocytes from 20 zebrafish (Y27632). Data are presented as mean ± SD. (e) Quantification of the number of mistargeted myelin profiles produced in oligodendrocytes which have not been demyelinated in control (median = 0.00, IQR = 0.00-0.00) and Y27632 ROCK inhibitor (median = 0.00, IQR = 0.00-0.00) treated zebrafish, p > 0.9999. Two-tailed Mann-Whitney test. N = 24 oligodendrocytes from 24 zebrafish (control), N = 20 oligodendrocytes from 20 zebrafish (Y27632). Data are presented as median with IQR. (f) Quantification of the number of sheaths produced per oligodendrocyte following demyelination in control (median = 3.00, IQR=1.50-3.00) and Y27632 ROCK inhibitor (median = 4.00, IQR = 1.00-4.50) treated zebrafish, p = 0.5964. Two-tailed Mann-Whitney test. N = 5 oligodendrocytes from 5 zebrafish (control), N = 9 oligodendrocytes from 9 zebrafish (Y27632). Data are presented as median with IQR. (g) Quantification of the number of mistargeted myelin profiles produced per oligodendrocyte following demyelination in control (median = 1.00, IQR = 1.00-2.00) and Y27632 ROCK inhibitor (median = 3.00, IQR = 1.50-4.75) treated zebrafish, p = 0.0414. Unpaired two tailed t-test with Welch’s correction. N = 5 oligodendrocytes from 5 zebrafish (control), N = 8 oligodendrocytes from 8 zebrafish (Y27632). Data are presented as mean ± SD.

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