Extended Data Fig. 6: Mistargeted myelin profiles are present in remyelinating lesions in motor cortex tissue from people with MS. | Nature Neuroscience

Extended Data Fig. 6: Mistargeted myelin profiles are present in remyelinating lesions in motor cortex tissue from people with MS.

From: New oligodendrocytes exhibit more abundant and accurate myelin regeneration than those that survive demyelination

Extended Data Fig. 6

(a) Low magnification image of chromogenic immunohistochemistry for proteolipid protein (PLP - brown) and NeuN (blue) in human MS motor cortex. Outline of quantified areas shown with lesion area highlighted in red, perilesion area highlighted in green, normal appearing grey matter (NAGM) in purple and white matter indicated by ‘WM’ in white. Images 1 and 2 show examples of quantified areas in 2 different human MS motor cortex samples. Scale bars, 2000 µm. (B and C) High magnification images of chromogenic immunohistochemistry for proteolipid protein (PLP - brown) and NeuN (blue) in human MS motor cortex. (b) Images 1-9 show example images of PLP+ve wrapped NeuN+ve cells (myelinated neuronal cell bodies). Images 1 and 3-9 scale bars, 20 µm. Image 2 scale bar, 10 µm. (c) Images 1-5 show example images of PLP + ve wrapped NeuN-ve cells (oligodendrocytes). Scale bars, 20 µm. (d) Fluorescent immunohistochemistry for NeuN (white), PLP (red) and Hoechst (nuclei-blue) in human MS motor cortex. Arrows indicate the location of PLP + ve wrapped NeuN+ve Hoechst+ve cells (myelinated neuronal cell body). Scale bar, 20 µm. (e) Images 1-5 show example images of CNPase+ve wrapped NeuN+ve cells (myelinated neuronal cell bodies). Scale bars, 20 µm. (f) Fluorescent immunohistochemistry for NeuN (green), CNPase (magenta) and Hoechst (nuclei-blue) in human MS motor cortex. Arrows indicate the location of CNPase+ve wrapped NeuN+ve Hoechst+ve cells. Scale bar, 20 µm.

Back to article page