Extended Data Fig. 7: Functional Responses of PVIN neurons.

a) Histogram of the maximum reliability score in all ROI (neurons) of each animal (thin lines) and group average (thick lines). The vertical line represent threshold for ROI to be considered visually responsive. b) Histogram of the average absolute response amplitude of all ROI (neurons) in each animal (thin lines) and group average (thick lines). c) Scatter plot representing location of all responsive cells in the tectum, color-coded by the position of the stimulus to which they responded most reliably. Although overall number of responsive cells is reduced in the ablation group, the retinotopy of the responses is preserved. d) Top: reliability scores of all responsive neurons for all stimuli, centered on the position of maximum responsiveness for each neuron and normalized to its maximum reliability. Dark gray line represents the average, light grey areas represent individual data points. Bottom: a gaussian line (red) was fit to each neuron’s responses (gray dots, data from an example neuron). The variance of the distribution was taken as a metric of the receptive field size of the neuron. The bar marks the 2 sigma interval from the sigmoidal fit of the reliability profile of an example cell. e) Histogram of the response curve variance of all ROI in each fish (thin lines) and group average (thick lines). (right) The average receptive field size was not significantly altered in the OPC ablated fish. Boxes represent median (center line) and I.Q.R. (1.541±1.329/1.633 in control vs. 1.590±1.306/1.861 in OPC-NTR ablation), the scatter dots represent the data for individual fish; two-tailed Mann–Whitney U test, U=−0.4308, n=12 MTZ-control animals and n=11 OPC-ablated animals from three experiments).